Optimization of Fermentation Conditions for Laccase Production by Recombinant Pichia pastoris GS115-LCCA Using Response Surface Methodology and Its Application to Dye Decolorization
نویسندگان
چکیده
منابع مشابه
Statistical Optimization of Conditions for Maximize Production of Mannan by Saccharomyces Cerevisiae Using Response Surface Methodology
In view of the increase in Saccharomyces cerevisiae mannan content, the culture condition for S.cerevisiae were optimized in this study. The influence of culture condition such as original pH, inoculum size, and temperature on mannan production were evaluated using Response surface methodology. The mathematical model was established by the quadratic rotary combination design. with the order of ...
متن کاملStatistical Optimization of Conditions for Maximize Production of Mannan by Saccharomyces Cerevisiae Using Response Surface Methodology
In view of the increase in Saccharomyces cerevisiae mannan content, the culture condition for S.cerevisiae were optimized in this study. The influence of culture condition such as original pH, inoculum size, and temperature on mannan production were evaluated using Response surface methodology. The mathematical model was established by the quadratic rotary combination design. with the order of ...
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The decolorization of direct Solophenyl red 3BL (SR), a polyazo dye extensively used in textile industry was studied. The Fomes fomentarius laccase alone did not decolorize SR. The natural redox mediator, acetosyringone (AS), was necessary for decolorization to occur. Box-Behnken design was used to evaluate the effects of three parameters, namely, enzyme concentration (0.5-2.5 U mL(-1)), redox ...
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results escherichia coli phytase was expressed in p. pastoris under different cultivation conditions (post-induction temperature, methanol concentration, and post-induction ph). the optimized conditions by rsm using face centered central composite design were 1% (v/v) methanol, ph = 5.8, and 24.5°c. under the optimized conditions, appa was successfully expressed in p. pastoris and the maximum p...
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conclusions it can be concluded that the rsm is an effective method for the optimization of recombinant protein expression using synthetic genes in e. coli. results the proposed optimum fermentation condition consisted of 7.81 g l-1 glucose, od 600 nm prior induction 1.66 and induction temperature of 30.27°c. the model prediction of 0.267 g l-1 of rhifn-β and 0.961 g l-1 of acetate at the optim...
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ژورنال
عنوان ژورنال: BioResources
سال: 2013
ISSN: 1930-2126
DOI: 10.15376/biores.8.3.4072-4087